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Specific fluorochromes for core (DNA) and surface (membrane) structures underline the characteristics of every particle, increasing the variety of clinical parameters can be determined.
A sheath reagent flow separates and aligns the particles in the sample to ensure their individual and proper measurement.
As each particle passes the 488nm-laser beam, a pattern of individual light signals is generated and captured by a set of detectors.
Detected light signal patterns are translated into individual 'fingerprints' allowing their identification and allocation to the according parameter. Ultimately, this leads to the quantification of each parameter.
All signals from one measurement are allocated to the corresponding parameters and summarised in scattergrams. Therefore each scattergram represents the signals of one sample for one parameter.